Part:BBa_K3408005:Design

Optimized DpnI

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1322
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 210
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We have made some improvement on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/lifescience/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html)to use it better in our engineering bacteria.

Source

The source of the part comes from BBA_K 526001 https://parts.igem.org/Part:BBa_K526001.